Variation in the cellular localization of host-protective oncospheral antigens in Taenia saginata and Taenia solium

A Jabbar; M Verástegui; JA Lackenby; AK Walduck; CG Gauci; RH Gilman; MW Lightowlers

Journal article

Variation in the cellular localization of host-protective oncospheral antigens in Taenia saginata and Taenia solium

A Jabbar, M Verástegui, JA Lackenby, AK Walduck, CG Gauci, RH Gilman, MW Lightowlers

Parasite Immunology | WILEY-BLACKWELL | Published : 2010

DOI: 10.1111/j.1365-3024.2010.01230.x

Abstract

Immunohistochemistry and immunofluorescence with confocal microscopy were used to localize the host-protective antigens of Taenia saginata (TSA9 and TSA18) and Taenia solium (TSOL16, TSOL18 and TSOL45). In nonactivated oncospheres, TSA9 and TSOL45 antigens were found primarily in the cytoplasm of the penetration gland type one (PG1) cell. A similar pattern of staining was seen for TSOL45 in oncospheres of T. solium that remained within the oncospheral membrane. In addition, there was less intense staining of TSA9 and TSOL45 in the quadri-nucleate penetration gland type 2 (PG2) cell. TSA18, TSOL16 and TSOL18 were predominantly found in the PG2 cell. In activated oncospheres that had escaped t..

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University of Melbourne Researchers

Abdul Jabbar Author

Charles Gauci Author

Marshall Lightowlers Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

The authors acknowledge the expert technical assistance of Mr Paul Benham as well as Faye Docherty. We are grateful to Mrs. Jacqueline Mills, Integrated Neuroscience Facility, Howard Florey Institute, Parkville, for her assistance in co-localization analysis. We are also thankful to Prof. Ian Beveridge for his comments to improve the quality of this manuscript. This work was supported by National Health and Medical Research Council (grants 400109 and 628320).